life sciences

Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

by NANOSENSORS
9 June 202015 March 2023
Science & Technology

Figure 4 a and b from Ana I. Gómez-Varela et al “Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences : Simultaneous SR-SIM/AFM acquisition. The AFM measurements were carried out on fixed U2OS cells in medium/buffer with (a) and without N-SIM illumination (b). For convenience and enhanced feature/noise contrast, both AFM topography images in the SR-SIM/AFM overlays are displayed with an edge detection algorithm using a pixel difference operator in X. The topography images from Petri dish surface on three positions (labelled in the figures) were planefit (1st order polynomial function) to compensate for tilts in the sample surface, and subjected to surface roughness analysis Please have a look at the full article to view the full figure. https://rdcu.be/b4Iot

Correlating data from different microscopy techniques holds the potential to discover new facets of signalling events in cellular biology.* In the article “Simultaneous co-localized super-resolution… Read More »Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices

by NANOSENSORS
30 March 202015 March 2023
Science & Technology

AFM images from Figure 6 from “DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices “ by Ming Liu et al.

MeCP2 and MBD2 are members of a family of proteins that possess a domain that selectively binds 5-methylcytosine in a CpG context. Members of the… Read More »DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices

Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

by NANOSENSORS
23 September 201915 March 2023
Science & Technology

The mechanical properties and structural organization of membranes determine the functional state of red blood cells (RBCs). Deformability is one of the key physiological and… Read More »Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

Substrate properties modulate cell membrane roughness by way of actin filaments

by NANOSENSORS
4 January 201915 March 2023
Science & Technology

Supplementary Figure S1 from Chao-Hung Chang et al. “Substrate properties modulate cell membrane roughness by way of actin filaments”: Images of membrane topography determined by atomic force microscopy (AFM). MEFs were seeded on the polymer coverslip-bottom μ-dishes coated with 0 or 10 μg/ml FN for 6 hours for the measurement of membrane roughness by AFM. The regions marked by the white squares in the bright-field images are displayed in the membrane topography. Scale bar, 10 μm. NANOSENSORS uniqprobe qp-SCONT AFM probes(long cantilever length 125 um, spring constant 0.01 N/m) were used.

“Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions”* In the article “Substrate properties modulate cell membrane roughness by… Read More »Substrate properties modulate cell membrane roughness by way of actin filaments

A fibrin biofilm covers blood clots and protects from microbial invasion

by NANOSENSORS
28 October 201815 March 2023
Science & Technology

Supplemental Figure. 6. From Macrea et. al “A fibrin biofilm covers blood clots and protects from microbial invasion” Mechanisms and roles of fibrin film. A, Sneddon model used to calculate Young’s Modulus, where F is the force from the force curve, E is Young’s modulus, ν is Poisson’s ratio (0.5), α is the half angle for the indenter (15 degrees for our tips), and δ is the indentation. Note that this equation is only accurate with a half angle of 15 degrees for the first 200nm of indentation. B, Strength of the fibrin film in clots produced with plasma and thrombin with or without T101 (FXIII inhibitor ) investigated using atomic force microscopy (AFM). Fibrin fibres were visible under the film surface and these areas presented with stiffer Young’s modulus than fibrin film suspended between fibres. Grey lines in the zoomed-in images represent Young’s modulus scan area represented in the line force graphs. Scale bar - 2μm. C, Young’s Modulus was calculated for the suspended film and the film supported by fibers with and without T101 by fitting a Sneddon model to all AFM force curves found over the entire area that was imaged. 20 measurements were taken for each condition. **** P

New interesting publication by Macrea et. al mentioning the use of NANOSENSORS uniqprobe qp-BioAC: “Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a… Read More »A fibrin biofilm covers blood clots and protects from microbial invasion

MAC Mode Cantilevers for Keysight, Agilent and Molecular Imaging AFMs now available from NANOSENSORS

by NANOSENSORS
17 September 201815 March 2023
News & Events

MAC mode imaging of lambda phage DNA in a buffer solution. Image courtesy of Keysight Technologies

Do you have a Keysight, Agilent or Molecular Imaging Atomic Force Microscope in your lab? Are you using MAC Mode (Keysight Technologies’ patented magnetic AC… Read More »MAC Mode Cantilevers for Keysight, Agilent and Molecular Imaging AFMs now available from NANOSENSORS

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils

by NANOSENSORS
15 August 201815 March 2023
Science & Technology

Figure 5 from Yordanova et. al. "Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils" - AFM image of the Zn2+: GnRH [6-D-Phe] 10:1 complex. (a,b) Oligomers after preparation with tapping mode in Tris buffer solution (c,d) fibrils with tapping mode in air (z-scale indicates the average size of the formed oligomers and fibrils). NANOSENSORS qp-BioAC AFM probe was used to perform images in buffer solution

For this article the AFM images taken with tapping mode in Tris buffer solution were performed with the NANOSENSORS qp-BioAC (cantilever 3, resonance frequency 30kHz).… Read More »Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils