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Accurate and rapid antibiotic susceptibility testing using a machine learning assisted nanomotion technology platform

Fig. 1 from Alexander Sturm et al. “Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform”: Nanomotion detection and recording platform. a Representation of the components of the nanomotion technology platform. b A representation of the nanomotion measurement setup with the (1) bacteria-loaded cantilever, (2) superluminescent light emitting diode (SLED) = light source, and (3) photodetector. c Schematic illustrating Gram-negative bacteria attached to the cantilever. Prior to attachment, bacteria are dispersed in gelling agarose while the cantilever surface is functionalized using positively charged poly-D-lysine. The gelling agent proved beneficial for an even distribution and stability of the bacterial attachment. d Representative standard 4-h nanomotion recordings with a 2-h medium phase (50% LB medium) followed by a 2-h drug phase with 32 µg/ml CRO for the E. coli reference strains ATCC-25922 (S, susceptible) and BAA-2452 (R, resistant). These recordings form the basis for using nanomotion to conduct AST. This study contains 219 recordings of ATCC-25922 and 225 recordings of BAA-2452 exposed to 32 µg/ml CRO with similar results. Data are available in the source data file. NANOSENSORSTM tipless uniqprobe AFM cantilevers SD-qp-CONT-TL from the NANOSENSORS Special Developments List were used.

Antimicrobial resistance (AMR) has become a significant threat to public health worldwide. * AMR diagnostic strategies such as antibiotic susceptibility testing (AST) help provide clinicians… Read More »Accurate and rapid antibiotic susceptibility testing using a machine learning assisted nanomotion technology platform

Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Figure 5 from “Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface” by Hassiba Smida et al. AFM images recorded in water of (A and C) CF and (B and D) Pt UME surfaces (A and B) before and (C and D) after a chronoamperometry measurement at +0.8 V vs Ag/AgCl in 2 mL of 20 mM K4Fe(CN)6 0.1 M PBS at pH 7.4 as aqueous solution under inert atmosphere (N2) in the presence of 109 cells of living SOMR1 bacteria sample. The circle in white dashed line is added for showing the UME disk on the AFM images. NANOSENSORS uniqprobe qp-SCONT AFM probes were used.

In the past few years, several novel analytical methods with high sensitivity and spatial-temporal resolution that enable qualitative and quantitative analysis at single-cell and subcellular… Read More »Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Figure 3 from “Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments” by Miguel Cantero et al.: Treatment of TGEV with IGEPAL 0.2% (A). Topographical images before (left) and after (right) IGEPAL treatment (B). Profiles traced over the particles before (black) and after (blue) the treatment. The time interval between images was ~30 s (C). Height distribution of TGEV particles before (black) and after (blue) treatment (n = 103). Counts taken from the distribution curve were normalized for comparison. The peak shifts from the value of the intact particle height to the height of the cores. NANOSENSORS uniqprobe qp-BioAC AFM probes were used for the atomic force microscopy measurements.

Successful airborne transmission of coronaviruses through fluid microdroplets requires a virion structure that must withstand harsh natural conditions. * Because of the strict biosafety requirements… Read More »Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments