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December 2022

Chemically characterizing the cortical cell nano-structure of human hair using atomic force microscopy integrated with infrared spectroscopy (AFM-IR)

Figure 2 from A. P. Fellows et al “Chemically characterizing the cortical cell nano-structure of human hair using atomic force microscopy integrated with infrared spectroscopy (AFM-IR)”: AFM-IR data obtained from a human hair cross-section (approx. 300 nm thick) showing (a) a schematic structural representation of the cortical cell (b) average spectra corresponding to each observed region (macrofibril and Ext-matrix) (c) AFM height map and (d)-(l) IR-intensity maps recorded in contact mode across a 2.5 × 2.5 μm region of the surface at a number of different infrared frequencies corresponding to contributions observed in the spectra (each shown above the corresponding image) for a representative fibre section. NANOSENSORS AdvancedTEC ATEC-CONTAu tip view AFM probes were used.

The structure of hair fibres has been the focus of much research over the last century with several techniques used to investigate their structure. *… 

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