Do you have a Keysight, Agilent or Molecular Imaging Atomic Force Microscope in your lab? Are you using MAC Mode (Keysight Technologies’ patented magnetic AC mode ) to image soft samples or for imaging in fluids?
Then NANOSENSORS™ has the right cantilevers for you.
For this article the AFM images taken with tapping mode in Tris buffer solution were performed with the NANOSENSORS qp-BioAC (cantilever 3, resonance frequency 30kHz).
Yordanka Yordanova, Willem Vanderlinden, Raphael Stoll, Daniel Rüdiger, Andreas Tosstorff, Wolfgang Zaremba, Gerhard Winter, Stefan Zahler & Wolfgang Friess Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils
Nature Scientific Reports volume 8, Article number: 11280 (2018)
Please follow this external link to read the full article on the Nature website: https://rdcu.be/4y9J
Abstract: A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+in vitro. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR–FTIR) revealed the existence of higher order assembly of Zn2+: GnRH [6-D-Phe]. Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+ and GnRH [6-D-Phe]. Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils. Molecular Dynamic (MD) simulation of the 10:1 Zn2+: GnRH [6-D-Phe] explored the interaction and dimerization processes. In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift. The lyophilized Zn2+: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices. The in vitro release was slow and continuous over 14 days and not influenced by the oil matrix.