Tag Archives: qp-SCONT

Soft, drift-reduced AFM cantilevers for Biology and Life Sciences – Uniqprobe Screencast passes the 1000 views mark

The screencast on the soft, drift-reduced NANOSENSORS™ uniqprobe cantilevers for biology and life science applications held by Dr. Laure Aeschimann has just passed the 1000 views mark. Congratulations Laure!

Since the first publication of this screencast that presents the uniqprobe types qp-BioAC, qp-BioT, qp-SCONT and qp-CONT , three further types of uniqprobe AFM probes have been introduced:

qp-BioAC-CI – a version of uniqprobe™ BioAC with Rounded Tips for Cell Imaging

qp-fast – three different uniqprobe™ cantilevers on one support chip for Soft- , Standard- , Fast Tapping/Dynamic AFM Imaging

and qp-HBC – the uniqprobe™ – HeartBeatCantilever that can also be used for ScanAsyst** and Peak Force Tapping** in Air.

To find out more please have a look at the NANOSENSORS™ uniqprobe brochure or the individual product pages on the NANOSENSORS webpage.

Additionally we have also put tipless versions of the qp-SCONT, qp-CONT and the qp-BioT ( SD-qp-BioT-TL, SD-qp-CONT-TL, SD-qp-SCONT-TL) and uniqprobe tipless cantilever arrays ( SD-qp-TL8a and SD-qp-TL8b ) on the NANOSENSORS special developments list.

Feel free to browse or let us know if you have any questions via info(at)nanosensors.com.

Product Screencast on the NANOSENSORS™ uniqprobe AFM Probes series with unsurpassed small variation in spring constant and resonance frequency by product developer Dr. Laure Aeschimann

A Japaneseversion of the screencast is also available :

バイオテクノロジー/ライフサイエンス向け NANOSENSORS ユニーク·プローブ Uniqprobe

A Chinese version of the Uniqprobe screencast is available on three different channels:

NANOSENSORS公司的吴烨娴博士在本视频中介绍了Uniqprobe原子力显微镜探针。Uniqprobe 探针 的悬臂梁厚度均一,并且有局部的金反射涂层。这两个特点使得这个探针在一些对弹性系数有精确要求的应用中, 表现出卓越的机械性能一致性 。该探针特别适用于分子生物学,生物物理学和定量纳米机械的研究.

The Chinese version is also available on Youku: http://v.youku.com/v_show/id_XNzA4MTgxNTI4.html
or weibo http://weibo.com/u/5077581192?is_all=1

** ScanAsyst® and PeakForce Tapping™ are trademarks of Bruker Corp.

Nucleation in confinement generates long-range repulsion between rough calcite surfaces

Fluid-induced alteration of rocks and mineral-based materials often starts at confined mineral interfaces where nm-thick water films can persist even at high overburden pressures and at low vapor pressures. These films enable transport of reactants and affect forces acting between mineral surfaces. However, the feedback between the surface forces and reactivity of confined solids is not fully understood.*

In “Nucleation in confinement generates long-range repulsion between rough calcite surfaces» Joanna Dziadkowiec, Bahareh Zareeipolgardani, Dag Kristian Dysthe and Anja Røyne describe how they used the surface forces apparatus (SFA) to follow surface reactivity in confinement and measure nm-range forces between two rough calcite surfaces in NaCl, CaCl2, or MgCl2 solutions with ionic strength of 0.01, 0.1 or 1 M.*

Roughness evolution with time of single, unconfined calcite films in salt solutions was analyzed by Atomic Force Microscopy using NANOSENSORS™ uniqprobe qp-SCONT AFM probes to image the surfaces in contact mode.*

 Supplementary Information S8. showing the Atomic Force Microscopy (AFM)ALD films roughness characterization from «Nucleation in confinement generates long-range repulsion between rough calcite surfaces” by Joanna Dziadkowiec et al.:
 Figure S7 show the AFM height maps (A, B, E, F, G, J) and histograms of surface heights (C, D, H, I) of the initial set 1 (A-E) and set 2 (F-J) ALD calcite surfaces for two scan sizes of 15x15 μm2(A, C, F, H)and 2x2 μm2(B, D, E, G, I, J). The images E and J show 3D height maps of the B, G height maps, respectively

Supplementary Information S8. Atomic Force Microscopy (AFM)ALD films roughness characterization from «Nucleation in confinement generates long-range repulsion between rough calcite surfaces” by Joanna Dziadkowiec et al.:
Figure S7.AFM height maps (A, B, E, F, G, J) and histograms of surface heights (C, D, H, I) of the initial set 1 (A-E) and set 2 (F-J) ALD calcite surfaces for two scan sizes of 15×15 μm2(A, C, F, H)and 2×2 μm2(B, D, E, G, I, J). The images E and J show 3D height maps of the B, G height maps, respectively

*Joanna Dziadkowiec, Bahareh Zareeipolgardani, Dag Kristian Dysthe and Anja Røyne
Nucleation in confinement generates long-range repulsion between rough calcite surfaces
Nature, Scientific Reports, volume 9, Article number: 8948 (2019)
doi: https://doi.org/10.1038/s41598-019-45163-6

Please follow this external link for the full article: https://rdcu.be/bMhZb

Open Access: The article “Nucleation in confinement generates long-range repulsion between rough calcite surfaces” by Joanna Dziadkowiec, Bahareh Zareeipolgardani, Dag Kristian Dysthe and Anja Røyne is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Substrate properties modulate cell membrane roughness by way of actin filaments

“Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions”*
In the article “Substrate properties modulate cell membrane roughness by way of actin filaments” Chao-Hung Chang, Hsiao-Hui Lee, and Chau-Hwang Lee employed the non-interferometric wide-field optical profilometry (NIWOP) technique to measure the membrane roughness of living mouse embryonic fibroblasts with different conditions of the culture substrate to find out whether membrane roughness is associated with the substrate properties. By controlling the surface density of fibronectin (FN) coated on the substrate, they found that cells exhibited higher membrane roughness as the FN density increased in company with larger focal adhesion (FA) sizes.

The examination of membrane roughness was also confirmed with atomic force microscopy.
The long cantilever of NANOSENSORS uniqprobe qp-SCONT AFM probes ( 125-μm long, spring constant 0.01 N/m.) was used to observe the membrane topography on living MEFs.

If you would like to learn more about the NANOSENSORS uniqprobe AFM probes series which offers soft, drift-reduced AFM probes with unsurpassed small variation in spring constant and resonance frequency mainly for biology and life science applications but also for other aplications such as high speed scanning then please have a look at our recently updated Uniqprobe brochure: https://www.nanosensors.com/pdf/NANOSENSORS-uniqprobe-brochure.pdf .

Supplementary Figure S1 from Chao-Hung Chang et al. “Substrate properties modulate cell membrane roughness by way of actin filaments”: Images of membrane topography determined by atomic force microscopy (AFM). MEFs were seeded on the polymer coverslip-bottom μ-dishes coated with 0 or 10 μg/ml FN for 6 hours for the measurement of membrane roughness by AFM. The regions marked by the white squares in the bright-field images are displayed in the membrane topography. Scale bar, 10 μm. NANOSENSORS uniqprobe qp-SCONT AFM probes(long cantilever length 125 um, spring constant 0.01 N/m) were used.
Supplementary Figure S1 from Chao-Hung Chang et al. “Substrate properties modulate cell membrane roughness by way of actin filaments”: Images of membrane topography determined by atomic force microscopy (AFM). MEFs were seeded on the polymer coverslip-bottom μ-dishes coated with 0 or 10 μg/ml FN for 6 hours for the measurement of membrane roughness by AFM. The regions marked by the white squares in the bright-field images are displayed in the membrane topography. Scale bar, 10 μm.

*Chao-Hung Chang, Hsiao-Hui Lee, Chau-Hwang Lee
Substrate properties modulate cell membrane roughness by way of actin filaments
Nature Scientific Reports, volume 7, Article number: 9068 (2017)
DOI: https://doi.org/10.1038/s41598-017-09618-y

Please follow this external link for the full article: https://rdcu.be/bdZm9

Open Access The article “Substrate properties modulate cell membrane roughness by way of actin filaments” by  Chao-Hung Chang et al. is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.