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Atomic Force Microscopy

Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation

Figure 5 from Catharina Husteden et al. 2023 “Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation”: A–D) Topography images [Cs/Col]4Cs (A), [Cs/Col]4Cs/LPX (B), [Cs/Col]4Cs/LPX/Cs (C), and [Cs/Col]4Cs/LPX/Cs/Col determined by AFM [Scale bar 1 µm] (D). E) Distribution curves of Young's modulus (E0) with a force map of a defined area (see also Figure S4, Supporting Information of the cited article). NANOSENSORSTM uniqprobe triangular qp-BioT AFM probes were used for the quantitative imaging to investigate the surface roughness and topography (in a standard liquid cell of a commercially available atomic force microscope) and force mapping. The uniqprobe qp-BioT AFM probe types offer an alternative to silicon nitride probes, with the advantage of taller AFM tips with smaller opening angles and reduced thermal drift.

The development of smart multifunctional biomaterials with the ability to control the behavior of stem cells on demand has become a powerful strategy in regenerative… Read More »Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation

Transparent electrodes based on molybdenum-titanium-oxide with increased water stability for use as hole-transport/hole-injection components

Figure 6 from Selina Goetz et al. 2022 “Transparent electrodes based on molybdenum–titanium–oxide with increased water stability for use as hole-transport/hole-injection components”: AFM images of a bare glass, b bare PET, c MTO40/Ag14/MTO10/30 on glass and d MTO40/Ag14/MTO10/30 on PET Figure 6 displays the 1 × 1 µm2 AFM images of the DMD on glass and PET, as well as the corresponding bare substrate. The roughness of the electrodes is mostly affected by the underlying substrate. The extracted root mean square value (RMS) for the glass substrate (Fig. 6a) is 1.8 ± 0.1 nm and 11.5 ± 4.0 nm for the PET substrate (Fig. 6b). The RMS values of the DMD electrodes are even reduced, yielding RMS = 1.0 ± 0.1 nm for glass/MTO40/Ag14/MTO10/30 (Fig. 6c) and RMS = 4.8 ± 2.0 nm for PET/MTO40/Ag14/MTO10/30 (Fig. 6d). NANOSENSORS silicon high-resolution AFM probes for tapping mode with a typical AFM tip radius of 2 nm ( SuperSharpSilicon™ SSS-NCHR ) were used.

The application of molybdenum oxide layers in electronic devices like solar cells and organic light emitting diodes (LEDs) has expanded considerably. * Dielectric/metal/dielectric (DMD) transparent… Read More »Transparent electrodes based on molybdenum-titanium-oxide with increased water stability for use as hole-transport/hole-injection components

Accurate and rapid antibiotic susceptibility testing using a machine learning assisted nanomotion technology platform

Fig. 1 from Alexander Sturm et al. “Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform”: Nanomotion detection and recording platform. a Representation of the components of the nanomotion technology platform. b A representation of the nanomotion measurement setup with the (1) bacteria-loaded cantilever, (2) superluminescent light emitting diode (SLED) = light source, and (3) photodetector. c Schematic illustrating Gram-negative bacteria attached to the cantilever. Prior to attachment, bacteria are dispersed in gelling agarose while the cantilever surface is functionalized using positively charged poly-D-lysine. The gelling agent proved beneficial for an even distribution and stability of the bacterial attachment. d Representative standard 4-h nanomotion recordings with a 2-h medium phase (50% LB medium) followed by a 2-h drug phase with 32 µg/ml CRO for the E. coli reference strains ATCC-25922 (S, susceptible) and BAA-2452 (R, resistant). These recordings form the basis for using nanomotion to conduct AST. This study contains 219 recordings of ATCC-25922 and 225 recordings of BAA-2452 exposed to 32 µg/ml CRO with similar results. Data are available in the source data file. NANOSENSORSTM tipless uniqprobe AFM cantilevers SD-qp-CONT-TL from the NANOSENSORS Special Developments List were used.

Antimicrobial resistance (AMR) has become a significant threat to public health worldwide. * AMR diagnostic strategies such as antibiotic susceptibility testing (AST) help provide clinicians… Read More »Accurate and rapid antibiotic susceptibility testing using a machine learning assisted nanomotion technology platform

Graphene removal by water-assisted focused electron-beam-induced etching – unveiling the dose and the dwell time impact on the etch profile and topographical changes in SiO2 substrates

Figure 4 from Aleksandra Szkudlarek et al 2024 “Graphene removal by water-assisted focused electron-beam-induced etching – unveiling the dose and dwell time impact on the etch profile and topographical changes in SiO2 substrates” : Results of in situ AFM measurements of the etched lines on the SiO2/Si substrate as a function of the dwell time: A) AFM profiles of lines etched with different dwell times (td = 1, 10, and100 μs); B) 3D AFM image of the SiO2 surface. The AFM-in-SEM approach allows for in situ analysis. Thus, the graphene could be modified and immediately measured by AFM without changing the environment. The analysis was performed by using the tapping mode and the NANOSENSORS self-sensing self-actuating Akiyama probe.

Graphene is one of the most extensively studied 2D materials, exhibiting extraordinary mechanical and electronic properties. * Although many years have passed since its discovery,… Read More »Graphene removal by water-assisted focused electron-beam-induced etching – unveiling the dose and the dwell time impact on the etch profile and topographical changes in SiO2 substrates