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Revealing the regulation of water dipole potential to aggregation of amyloid-β at chiral interface by surface-enhanced infrared adsorption spectroscopy

Figure 3 from Manyu Zhu et al. (2024), “Revealing the regulation of water dipole potential to aggregation of amyloid-β 42 at chiral interface by surface-enhanced infrared absorption spectroscopy” Aggregation of Aβ42 on different chiral interfaces. (A–E) AFM images of L-NIBC surface and (H–L) D-NIBC surface after incubation in Aβ42 monomer solution for 0 min, 10 min, 1 h, 12 h, 24 h at 37°C in the presence of 10 mm PBS at pH 7.4. (F,M) AFM images of L-NIBC surface and D-NIBC surface after incubation in Aβ42 monomer solution in the presence of 10 mm PBS at pH 9.0. (G,N) AFM images of L-NIBC surface and D-NIBC surface after incubation in Aβ42 monomer solution in the presence of 10 mm PBS and 150 mm NaCl at pH 7.4. (O,P) Time-dependent SEIRA spectra of Aβ42 on L-NIBC interface and on D-NIBC interface. (Q) Time-dependent surface coverage of Aβ42 on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS at pH 7.4, which was calculated by integrating the area of the amide II band. (R) Integrated area of amide II band on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. (S) Amide I band peak position on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. (T) Integrated area of amide II band on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS and 150 mm NaCl at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. (U) Amide I band peak position on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS and 150 mm NaCl at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. The morphology of Aβ42 was measured with Atomic Force Microscopy (AFM) in tapping mode using NANOSENSORS PointProbe® Plus PPP-NCHR AFM probes.

Surface chirality plays an important role in determining the biological effect. Understanding how surface chirality regulates the behaviors of biological entities such as biomolecules and… Read More »Revealing the regulation of water dipole potential to aggregation of amyloid-β at chiral interface by surface-enhanced infrared adsorption spectroscopy

Mechanical disassembly of human picobirnavirus like particles indicates that cargo retention is tuned by the RNA-coat protein interaction

Fig. 6 from María J. Rodríguez-Espinosa et al. 2023: Model depicting the RNA cargo retention for each VLP variant. The Δ45-CP VLP structure (A) does not show externalized cargo. The CP VLP structure (B) shows shorter N-terminals compared to the Ht-CP structure (C), which implies less RNA cargo retention compared to the Ht-CP structure. The cartoons in blue, green and pink colors represent coat protein, RNA and N-terminal, respectively. For mechanical fatigue experiments, rectangular AFM cantilevers (NANOSENSORS™, uniqprobe qp-BioAC AFM probes with three different AFM cantilevers per chip) with nominal spring constants of 0.05 and 0.1 N m−1 were used. The AFM cantilevers were calibrated using Sader's method.

The idea of using virus-like particles as nanocarriers for heterologous cargo transport and delivery requires controlling the stability of the container–cargo system.* In particular, the… Read More »Mechanical disassembly of human picobirnavirus like particles indicates that cargo retention is tuned by the RNA-coat protein interaction

A Bis(terpyridine)nickel(II)-Based Coordination Nanosheet: A Redox-Active Materials with Flexibility and Transparency

Fig. 2f from Kenji Takada et al. 2024 “A Bis(terpyridine)nickel(II)-Based Coordination Nanosheet: A Redox-Active Material with Flexibility and Transparency” Synthesis and morphology identification of 1-Ni. f Topographic AFM image of 1-Ni on a flat Si substrate and the cross-sectional analysis inset For the full figure please refer to the cited article. NANOSENSORS PointProbe Plus® PPP-NCL AFM probes were used for the characterization with atomic force microscopy.

Since the discovery of graphene, extensive studies have revealed various types of novel two-dimensional polymers such as transition metal dichalcogenides, covalent organic frameworks, and carbon… Read More »A Bis(terpyridine)nickel(II)-Based Coordination Nanosheet: A Redox-Active Materials with Flexibility and Transparency