quantitative imaging

Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Figure 5 from “Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface” by Hassiba Smida et al. AFM images recorded in water of (A and C) CF and (B and D) Pt UME surfaces (A and B) before and (C and D) after a chronoamperometry measurement at +0.8 V vs Ag/AgCl in 2 mL of 20 mM K4Fe(CN)6 0.1 M PBS at pH 7.4 as aqueous solution under inert atmosphere (N2) in the presence of 109 cells of living SOMR1 bacteria sample. The circle in white dashed line is added for showing the UME disk on the AFM images. NANOSENSORS uniqprobe qp-SCONT AFM probes were used.

In the past few years, several novel analytical methods with high sensitivity and spatial-temporal resolution that enable qualitative and quantitative analysis at single-cell and subcellular… Read More »Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability

by NANOSENSORS
3 February 202115 March 2023
Science & Technology

Figure 4 from “Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability” by Cyriel Sebastiaan Olie et al. Disrupted cytoskeletal spatial organization in shPAB human muscle cell cultures. A Representative images of control and shPAB human muscle cell cultures, stained with antibodies to tubulin and actin, and the actin filaments were visualized with actin-GFP. B Tubulin staining in control and shPAB myoblast cell cultures after DMSO, 100 nM nocodazole or 25 nM paclitaxel treatment for 2 h. Scale bar is 25 µm. C Measurements of cell-mechanics properties in control and shPAB cells using the Brillouin Light Scattering Microscopy (Ci) or the Atomic Force Microscopy (Cii). Measurements were carried out in myoblasts; every dot represents the median from 1000 measurements in a cell. Cell stiffness is measured by GHz, and the young modulus reports the cell surface tension. Averages and standard deviations are from N = 15 cells. Statistical significance was calculated with the student’s t-test. NANOSENSORS uniqprobe qp-BioAC ( CB3 ) AFM probes were used in the quantitative imaging of cell-mechanics properties where a force curve is applied at each point.

Muscle wasting is connected with changes in various cellular mechanisms that influence protein homeostasis, transcription, protein acetylation and different metabolic pathways. * Scientific studies have… Read More »Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability