The NANOSENSORS screencast on the calibration service for AFM cantilevers has just reached the 1000 views mark.
Accurately determined cantilever properties are very important for
quantitative force measurements. Force constant and resonance frequency
are determined either by thermal tune, the Sader- or the dimensional
method, respectively. Usually, the thermal tune method delivers the most
precise values, but suffers from the fact that the AFM tip has to get
in contact with the surface to calibrate the photo-detector sensitivity.
This procedure may damage or break the tip!
NANOSENSORS™ offers a thermal tune calibration procedure performed by
Laser vibrometry. This method is contact free and therefore does no
damage the tip, but preserves the original AFM tip quality. To ensure
the highest level of accuracy NANOSENSORS™ cantilever calibration method
is calibrated with a standard, certified by the national German
“Animal cells undergo rapid rounding during mitosis, ensuring proper chromosome segregation, during which an outward rounding force abruptly increases upon prometaphase entry and is maintained at a constant level during metaphase. Initial cortical tension is generated by the actomyosin system to which both myosin motors and actin network architecture contribute. However, how cortical tension is maintained and its physiological significance remain unknown.”*
In their publication “Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase” Koutarou Nishimura et al. describe the uncovering of a previously unknown mechanism by which Cdk1 coordinates cortical tension maintenance and SAC inactivation at anaphase onset.*
Open Access The article “Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase” by Koutarou Nishimura et al. is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
New interesting publication by Macrea et. al mentioning the use of NANOSENSORS uniqprobe qp-BioAC:
“Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The fibrin network is porous and highly permeable, but the spatial arrangement of the external clot face is unknown. Here we show that fibrin transitioned to the blood-air interface through Langmuir film formation, producing a protective film confining clots in human and mouse models. We demonstrated that only fibrin is required for formation of the film, and that it occurred in vitro and in vivo. The fibrin film connected to the underlying clot network through tethering fibers. It was digested by plasmin, and formation of the film was prevented with surfactants. Functionally, the film retained blood cells and protected against penetration by bacterial pathogens in a murine model of dermal infection. Our data show a remarkable aspect of blood clotting in which fibrin forms a protective film covering the external surface of the clot, defending the organism against microbial invasion.”*
The AFM imaging and force measurements mentioned in this article were performed using CB3 of the NANOSENSORS™ uniqprobeqp-BioAC.
*Fraser L. Macrae, Cédric Duval, Praveen Papareddy, Stephen R. Baker, Nadira Yuldasheva, Katherine J. Kearney, Helen R. McPherson, Nathan Asquith, Joke Konings, Alessandro Casini, Jay L. Degen, Simon D. Connell, Helen Philippou, Alisa S. Wolberg, Heiko Herwald, Robert A.S. Ariëns A fibrin biofilm covers blood clots and protects from microbial invasion
Journal of Clinical Investigation. 2018;128(8):3356-3368
The article “A fibrin biofilm covers blood clots and protects from microbial invasion” by Fraser L. Macrae et. al is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.