applied chemistry

Revealing the regulation of water dipole potential to aggregation of amyloid-β at chiral interface by surface-enhanced infrared adsorption spectroscopy

Figure 3 from Manyu Zhu et al. (2024), “Revealing the regulation of water dipole potential to aggregation of amyloid-β 42 at chiral interface by surface-enhanced infrared absorption spectroscopy” Aggregation of Aβ42 on different chiral interfaces. (A–E) AFM images of L-NIBC surface and (H–L) D-NIBC surface after incubation in Aβ42 monomer solution for 0 min, 10 min, 1 h, 12 h, 24 h at 37°C in the presence of 10 mm PBS at pH 7.4. (F,M) AFM images of L-NIBC surface and D-NIBC surface after incubation in Aβ42 monomer solution in the presence of 10 mm PBS at pH 9.0. (G,N) AFM images of L-NIBC surface and D-NIBC surface after incubation in Aβ42 monomer solution in the presence of 10 mm PBS and 150 mm NaCl at pH 7.4. (O,P) Time-dependent SEIRA spectra of Aβ42 on L-NIBC interface and on D-NIBC interface. (Q) Time-dependent surface coverage of Aβ42 on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS at pH 7.4, which was calculated by integrating the area of the amide II band. (R) Integrated area of amide II band on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. (S) Amide I band peak position on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. (T) Integrated area of amide II band on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS and 150 mm NaCl at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. (U) Amide I band peak position on L (blue)/D-NIBC (red) chiral interfaces in the presence of 10 mm PBS and 150 mm NaCl at pH 5.0, 6.0, 7.4, 8.0, 9.0, respectively. The morphology of Aβ42 was measured with Atomic Force Microscopy (AFM) in tapping mode using NANOSENSORS PointProbe® Plus PPP-NCHR AFM probes.

Surface chirality plays an important role in determining the biological effect. Understanding how surface chirality regulates the behaviors of biological entities such as biomolecules and… Read More »Revealing the regulation of water dipole potential to aggregation of amyloid-β at chiral interface by surface-enhanced infrared adsorption spectroscopy