Successful airborne transmission of coronaviruses through fluid microdroplets requires a virion structure that must withstand harsh natural conditions. *
Because of the strict biosafety requirements for the study of human respiratory viruses, it is important to develop surrogate models to facilitate their investigation. *
In the article “Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments” Miguel Cantero, Diego Carlero, Francisco Javier Chichón, Jaime Martín-Benito and Pedro José De Pablo explore the mechanical properties and nanostructure of transmissible gastroenteritis virus (TGEV) virions in liquid milieu and their response to different chemical agents commonly used as biocides in their quest for a SARS-CoV2 surrogate for dynamic nanoscale structure studies that can alleviate the use of BSL3 labs that are highly demanded for biomedical and biotechnological research. *
In past few years, atomic force microscopy (AFM) has been used to thoroughly characterize the physical properties, structure and stability of many viruses. *
It is possible to scan individual viruses, obtaining their topography and a variety of physical properties such as mechanics or electrostatics in controlled liquid milieu. Atomic Force Microscopy has provided biophysical information on all kinds of viruses, including bacteriophages and eukaryotic viruses. *
For the research described in their article the authors used AFM to explore in real time the stability of individual TGEV particles as a surrogate model for SARS-CoV-2 in order to elucidate its structural stability under a range of physicochemical assaults, including mechanical stress, desiccation-rehydration cycles and treatment with chemical agents commonly used as biocides, such as detergents and ethanol. *
They also aimed to show that some structural research can be performed with non-hazardous CoV strains. *
All the described AFM experiments were carried out with NANOSENSORS™ uniqprobe qp-BioAC AFM probes. *
The data collected by Miguel Cantero et al. for the article provide two-fold results on virus stability:
First, while particles with larger size and lower packing fraction kept their morphology intact after successive mechanical aggressions, smaller viruses with higher packing fraction showed conspicuous evidence of structural damage and content release.
Second, monitoring the structure of single TGEV particles in the presence of detergent and alcohol in real time revealed the stages of gradual degradation of the virus structure in situ. *
These data suggest that detergent is three orders of magnitude more efficient than alcohol in destabilizing TGEV virus particles, paving the way for optimizing hygienic protocols for viruses with similar structure, such as SARS-CoV-2. *
*Miguel Cantero, Diego Carlero, Francisco Javier Chichón, Jaime Martín-Benito and Pedro José De Pablo
Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments
Cells 2022, 11(11), 1759
Open Access: The article “Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments” by Miguel Cantero, Diego Carlero, Francisco Javier Chichón, Jaime Martín-Benito and Pedro José De Pablo is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.