Tag Archives: 原子力显微镜

Development of a Lidocaine-Loaded Alginate/CMC/PEO Electrospun Nanofiber Film and Application as an Anti-Adhesion Barrier

Surgery, particularly open surgery, is known to cause tissue/organ adhesion during healing. These adhesions occur through contact between the surgical treatment site and other organ, bone, or abdominal sites. Fibrous bands can form in unnecessary contact areas and cause various complications. Consequently, film- and gel-type anti-adhesion agents have been developed. The development of sustained drug delivery systems is very important for disease treatment and prevention.*

In “Development of a Lidocaine-Loaded Alginate/CMC/PEO Electrospun Nanofiber Film and Application as an Anti-Adhesion Barrier” Seungho Baek, Heekyung Park, Youngah Park, Hyun Kang and Donghyun Lee describe how the drug release behavior was controlled by crosslinking lidocaine-loaded alginate/carboxymethyl cellulose (CMC)/polyethylene oxide (PEO) nanofiber films prepared by electrospinning.*

Lidocaine is mainly used as an anesthetic and is known to have anti-adhesion effects.*

Based on the results presented in the article, this study shows that the drug release behavior can be controlled by using CaCl2 as a nontoxic crosslinking agent to produce a good anti-adhesion barrier that can prevent unnecessary tissue adhesion at a surgical site.*

The authors selected atomic force microscopy (AFM) using NANOSENSORS™ PointProbe® Plus PPP-NCHR AFM cantilevers to analyze the electrospun films.*

Figure 3 from “Development of a Lidocaine-Loaded Alginate/CMC/PEO Electrospun Nanofiber Film and Application as an Anti-Adhesion Barrier” by Seungho Baek et al.:
Morphological and surface characterization of the 9% (w/v) alginate/CMC/PEO nanofiber film. Analyses used the noncontact mode of atomic microscopy. (a–c) are the same films at different scales (scale bars 40 µm, 15 µm, and 5 µm).
Figure 3 from “Development of a Lidocaine-Loaded Alginate/CMC/PEO Electrospun Nanofiber Film and Application as an Anti-Adhesion Barrier” by Seungho Baek et al.:
Morphological and surface characterization of the 9% (w/v) alginate/CMC/PEO nanofiber film. Analyses used the noncontact mode of atomic microscopy. (a–c) are the same films at different scales (scale bars 40 µm, 15 µm, and 5 µm).

*Seungho Baek, Heekyung Park, Youngah Park, Hyun Kang and Donghyun Lee
Development of a Lidocaine-Loaded Alginate/CMC/PEO Electrospun Nanofiber Film and Application as an Anti-Adhesion Barrier
Polymers 2020, 12(3), 618
DOI: https://doi.org/10.3390/polym12030618

Please follow this external link to read the full article: https://www.mdpi.com/2073-4360/12/3/618/htm

Open Access: The article “Development of a Lidocaine-Loaded Alginate/CMC/PEO Electrospun Nanofiber Film and Application as an Anti-Adhesion Barrier” by Seungho Baek, Heekyung Park, Youngah Park, Hyun Kang and Donghyun Lee is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

Correlating data from different microscopy techniques holds the potential to discover new facets of signalling events in cellular biology.*

In the article “Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences” Ana I. Gómez-Varela, Dimitar R. Stamov, Adelaide Miranda, Rosana Alves, Cláudia Barata-Antunes, Daphné Dambournet, David G. Drubin, Sandra Paiva and Pieter A. A. De Beule report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit.*

The authors detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.*

The simultaneous operation of AFM and super-resolution fluorescence microscopy technique provides a powerful observational tool on the nanoscale, albeit data acquisition is typically obstructed by a series of integration problems. The authors of the above-mentioned article believe that the combination of SR-SIM with AFM presents one of the most promising schemes enabling simultaneous co-localized imaging, allowing the recording of nanomechanical data and cellular dynamics visualization at the same time.*

For measurements on cells in liquid NANOSENSORS™ uniqprobe qp-BioAC-CI AFM probes ( CB1 ) with a nominal resonance frequency of 90 kHz (in air), spring constant of 0.3 Nm−1, partial gold coating on the detector side, and quartz-like circular symmetric hyperbolic (double-concaved) tips with ROC of 30 nm were used. The corresponding AFM areas for the cell images were acquired with a Z-cantilever velocity of 250 μms−1 at a max Z-length of 1.5 μm, resulting in an acquisition time (based on the number of pixels) for Figs. 2, 3, 4 of ca. 13, 8 and 15 min respectively.*

Figure 4 a and b from Ana I. Gómez-Varela et al “Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences :  Simultaneous SR-SIM/AFM acquisition. The AFM measurements were carried out on fixed U2OS cells in medium/buffer with (a) and without N-SIM illumination (b). For convenience and enhanced feature/noise contrast, both AFM topography images in the SR-SIM/AFM overlays are displayed with an edge detection algorithm using a pixel difference operator in X. The topography images from Petri dish surface on three positions (labelled in the figures) were planefit (1st order polynomial function) to compensate for tilts in the sample surface, and subjected to surface roughness analysis Please have a look at the full article to view the full figure. https://rdcu.be/b4Iot
Figure 4 a and b from Ana I. Gómez-Varela et al “Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences : Simultaneous SR-SIM/AFM acquisition. The AFM measurements were carried out on fixed U2OS cells in medium/buffer with (a) and without N-SIM illumination (b). For convenience and enhanced feature/noise contrast, both AFM topography images in the SR-SIM/AFM overlays are displayed with an edge detection algorithm using a pixel difference operator in X. The topography images from Petri dish surface on three positions (labelled in the figures) were planefit (1st order polynomial function) to compensate for tilts in the sample surface, and subjected to surface roughness analysis. Please have a look at the full article to view the full figure. https://rdcu.be/b4Iot

*Ana I. Gómez-Varela, Dimitar R. Stamov, Adelaide Miranda, Rosana Alves, Cláudia Barata-Antunes, Daphné Dambournet, David G. Drubin, Sandra Paiva and Pieter A. A. De Beule
Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
Nature Scientific Reports volume 10, Article number: 1122 (2020)
DOI: https://doi.org/10.1038/s41598-020-57885-z

Please follow this external link to read the full article https://rdcu.be/b4Iot

Open Access: The article “Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences” by Ana I. Gómez-Varela, Dimitar R. Stamov, Adelaide Miranda, Rosana Alves, Cláudia Barata-Antunes, Daphné Dambournet, David G. Drubin, Sandra Paiva and Pieter A. A. De Beule is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Nontoxic pyrite iron sulfide nanocrystals as second electron acceptor in PTB7:PC71BM-based organic photovoltaic cells

Iron disulfide ( FeS2 ) is a natural earth-abundant and nontoxic material with possible applications in lithium batteries, transistors or photovoltaic (PV) devices. According to the analysis carried out by Wadia et al., among 23 semiconducting materials, FeS2 is the best candidate for the development of large-scale solar cells at low cost (<2 × 10−6 ¢/W). Furthermore, FeS2 exhibits excellent optoelectronic properties such as a band gap of 0.8 to 1.38 eV, a high optical absorption coefficient (2 × 105 cm−1), high carrier mobility (2 to 80 cm2/Vs) and a large charge carrier lifetime (200 ps). Therefore, FeS2 nanoparticles (NPs) can be a good alternative for PV applications.*

In “Nontoxic pyrite iron sulfide nanocrystals as second electron acceptor in PTB7:PC71BM-based organic photovoltaic cells “ Olivia Amargós-Reyes, José-Luis Maldonado, Omar Martínez-Alvarez, María-Elena Nicho, José Santos-Cruz, Juan Nicasio-Collazo, Irving Caballero-Quintana and Concepción Arenas-Arrocena report the synthesis of nontoxic pyrite iron sulfide ( FeS2 ) nanocrystals (NCs) using a two-pot method. Moreover, they study the influence of these NCs incorporated into the PTB7:PC71BM active layer of bulk-heterojunction ternary organic photovoltaic ( OPV ) cells.*

The AFM roughness images presented in this article were acquired in dynamic force mode using NANOSENSORS™ PointProbe® Plus PPP-NCLAu AFM probes.

Figure 7 from “Nontoxic pyrite iron sulfide nanocrystals as second electron acceptor in PTB7:PC71BM-based organic photovoltaic cells” shows the 2D (left) and 3D (right) AFM images of the OPVs with different concentrations of FeS2 recorded in the noncontact mode. The roughness of the OPV surface is increased gradually as the FeS2 concentration increases (Table 1 and Figure 7), such that traps for the charge carriers could occur and the leakage current could increase. Because of the FeS2 agglomerates, the OPV parameters tend to decrease, free charges cannot be efficiently extracted. This effect is most prominent for the OPV cells with 1% of FeS2 (Figure 7 and Supporting Information File 1, Figure S2d).
Figure 7 from “Nontoxic pyrite iron sulfide nanocrystals as second electron acceptor in PTB7:PC71BM-based organic photovoltaic cells” by Olivia Amargós-Reyes et al.:
2D (left) and 3D (right) AFM images of the OPVs with different concentrations of FeS2
(a) 0.0 wt %, b) 0.25 wt %, c) 0.5 wt % and d) 1.0 wt %) recorded in noncontact mode.

*Olivia Amargós-Reyes, José-Luis Maldonado, Omar Martínez-Alvarez, María-Elena Nicho, José Santos-Cruz, Juan Nicasio-Collazo, Irving Caballero-Quintana and Concepción Arenas-Arrocena
Nontoxic pyrite iron sulfide nanocrystals as second electron acceptor in PTB7:PC71BM-based organic photovoltaic cells
Beilstein Journal of . Nanotechnology 2019, 10, 2238–2250.
DOI: doi:10.3762/bjnano.10.216

Please follow this external link to read the full article: https://www.beilstein-journals.org/bjnano/articles/10/216#R65

Open Access: The article “Nontoxic pyrite iron sulfide nanocrystals as second electron acceptor in PTB7:PC71BM-based organic photovoltaic cells” by Olivia Amargós-Reyes, José-Luis Maldonado, Omar Martínez-Alvarez, María-Elena Nicho, José Santos-Cruz, Juan Nicasio-Collazo, Irving Caballero-Quintana and Concepción Arenas-Arrocena is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.