uniqprobe™ BioAC with Rounded Tips for Cell Imaging

NANOSENSORS qp-BioAC-CI AFM probes are based on NANOSENSORS qp-BioAC probes.
For the qp-BioAC-CI type the AFM tips have been rounded to a nominal tip radius of 30nm for Cell Imaging applications. This probe is dedicated to measurements on soft and life science samples only.

These probes feature three different rectangular cantilevers on one side of the support chip.
The uniqprobe BioAC cantilevers unite fairly high resonance frequencies with low force constants.

The reflex gold coating deposited on the detector side of the cantilever covers only the free end above where the tip is located. Main advantages of the uniqprobe coating are considerably less cantilever bending and reduced drift particularly for measurements in liquid environments.

The NANOSENSORS uniqprobe combines the well-known features of the other NANOSENSORS AFM probe series such as high application versatility and compatibility with most commercial SPMs with the additional advantage of a strongly reduced dispersion of force constant and resonance frequency.

The unsurpassed uniformity of the mechanical characteristics of the uniqprobe series is particularly important for applications, where a large number of probes with known and near identical force constants or resonance frequencies are needed. The AFM probes of the uniqprobe series are especially adapted for molecular biology, biophysics and quantitative nano-mechanical studies.

The probe offers unique features:

  • typical tip radius of curvature about 30nm
  • circular symmetric tip shape with a hyperbolic profile
  • typical tip height 7µm
  • stress free cantilevers
  • reduced drift for applications in liquid environments
  • small dispersion of force constant and resonance frequency
  • tip and cantilevers are made of a quartz-like material
  • alignment grooves on backside of silicon holder chip
  • tip repositioning accuracy of better than ± 8µm (in combination with Alignment Chip)
  • chemically inert
This AFM probe features alignment grooves on the back side of the holder chip. These grooves fit to the NANOSENSORS Alignment Chip.

How to optimize AFM scanning parameters: list-img
Cantilever data:
Property Nominal Value Specified Range
Resonance Frequency [kHz] CB1: 90
CB2: 50
CB3: 30
CB1: 65 - 115
CB2: 35 - 65
CB3: 24 - 36
Force Constant [N/m] CB1: 0.3
CB2: 0.1
CB3: 0.06
CB1: 0.15 - 0.55
CB2: 0.06 - 0.18
CB3: 0.03 - 0.09
Length [µm] CB1: 40
CB2: 60
CB3: 80
CB1: 35 - 45
CB2: 55 - 65
CB3: 75 - 85
Mean Width [µm] CB1: 20
CB2: 25
CB3: 30
CB1: 18 - 22
CB2: 23 - 27
CB3: 28 - 32
Thickness [nm] CB1: 400
CB2: 400
CB3: 400
CB1: 370 - 430
CB2: 370 - 430
CB3: 370 - 430
Order codes and shipping units:
Order Code AFM probes per pack
qp-BioAC-CI-10 10
qp-BioAC-CI-20 20
qp-BioAC-CI-50 50

Special handling information for NANOSENSORS™ uniqprobes

Due to their unique geometry the tips of the uniqprobes are more susceptible to tip damage by electrostatic discharge (ESD) than other Silicon-SPM-Probes.

Electric fields near the probe chip may lead to field evaporation which can blunt the tip apex of the probe tip. Therefore the NANOSENSORS™ uniqprobes are shipped in specially designed ESD-safe chip carriers.

NANOSENSORS™ recommends to their customers to take appropriate precautions to avoid tip damage due to electrostatic discharge when handling the probes. This can for example be done by using anti-electrostatic mats, wrist bands and tweezers.

NANOSENSORS™ uniqprobe AFM Probes series