{"id":4481,"date":"2021-11-20T17:05:19","date_gmt":"2021-11-20T15:05:19","guid":{"rendered":"https:\/\/nanosensors.com\/blog\/supercritical-carbon-dioxide-decellularization\/"},"modified":"2023-03-15T14:49:50","modified_gmt":"2023-03-15T12:49:50","slug":"supercritical-carbon-dioxide-decellularization","status":"publish","type":"post","link":"https:\/\/www.nanosensors.com\/blog\/supercritical-carbon-dioxide-decellularization\/","title":{"rendered":"Supercritical carbon dioxide decellularization &#8230; !!!"},"content":{"rendered":"<p>Biocompatible scaffolds that can be repopulated with human cells have many uses such serving as replacement organs and tissues. Therefore there is an increasing interest in plant-based biomaterials for tissue engineering.*<\/p>\n<p><!-- \/wp:post-content --><!-- wp:paragraph --><\/p>\n<p>As the above mentioned scaffolds should mimic the in vivo tissue environment closely they need to provide a fitting structural and biomechanical support to the cells while at the same time promoting cell behaviour and tissue development. *<\/p>\n<p><!-- \/wp:paragraph --><!-- wp:paragraph --><\/p>\n<p>Currently the standard method to prepare plant tissue to serve as a biocompatible scaffold is to decellularize it with serial chemical treatment.*<\/p>\n\n<!-- wp:image {\"id\":4479,\"sizeSlug\":\"large\",\"linkDestination\":\"media\"} -->\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"573\" src=\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe-1024x573.jpg\" alt=\"Figure 3 from \u201cSupercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds AFM imaging and spectrometry measurement\u201d by Ashlee F. Harris et al.: They used AFM surface topography measurements to confirm that the structures such as plant vasculature were preserved after the scSO2 process and used AFM force curves to calculate the Young\u2019s Modulus (YM) of the scCO2 decellularized scaffold. NANOSENSORS uniqprobe qp-BioAC AFM probes were used for the described AFM measurments. (a) Representative false colored three-dimensional surface mapping images and (b) Young\u2019s modulus of scCO2 and chemically decellularized scaffolds (data as mean\u2009\u00b1\u2009SEM; n\u2009=\u20095).\" class=\"wp-image-4479\" srcset=\"https:\/\/www.nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe-1024x573.jpg 1024w, https:\/\/www.nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe-300x168.jpg 300w, https:\/\/www.nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe-768x429.jpg 768w, https:\/\/www.nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe-563x315.jpg 563w, https:\/\/www.nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-3-from-A-Harris-et-al-Supercritical-carbon-dioxide-decellularization-of-plant-material-to-generate-3D-biocompatible-scaffolds-qp-BioAC-AFM-probe.jpg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption class=\"wp-element-caption\">Figure 3 from \u201cSupercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds AFM imaging and spectrometry measurement\u201d by Ashlee F. Harris et al.: (a) Representative false colored three-dimensional surface mapping images and (b) Young\u2019s modulus of scCO2 and chemically decellularized scaffolds (data as mean\u2009\u00b1\u2009SEM; n\u2009=\u20095).<\/figcaption><\/figure>\n<!-- \/wp:image -->","protected":false},"excerpt":{"rendered":"<p>Biocompatible scaffolds that can be repopulated with human cells have many uses such serving as replacement organs and tissues. Therefore there is an increasing interest in plant-based biomaterials for tissue engineering.* As the above mentioned scaffolds should mimic the in vivo tissue environment closely they need to provide a fitting structural and biomechanical support to&hellip;&nbsp;<a href=\"https:\/\/www.nanosensors.com\/blog\/supercritical-carbon-dioxide-decellularization\/\" class=\"\" rel=\"bookmark\">Read More &raquo;<span class=\"screen-reader-text\">Supercritical carbon dioxide decellularization &#8230; !!!<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":4479,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[8],"tags":[],"class_list":["post-4481","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nanosensors-news"],"_links":{"self":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4481","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/comments?post=4481"}],"version-history":[{"count":0,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4481\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media\/4479"}],"wp:attachment":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media?parent=4481"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/categories?post=4481"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/tags?post=4481"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}