The authors used special NANOSENSORS\u2122 PointProbe\u00ae Plus<\/a> PPP-BSI<\/a> AFM probes for the single-molecule force tracing.<\/p>\n\n\n\n The NANOSENSORS PPP-BSI AFM tips were modified in two steps as described in their article: First they were washed by Piranha solution (V(H2SO4):V(H2O2) = 3:1) for\u00a0 1\u00a0 h,\u00a0 cleaned\u00a0 with\u00a0 ultrapure\u00a0 water\u00a0 twice\u00a0 and\u00a0 absolute\u00a0 ethylacohol\u00a0 once\u00a0 then the AFM tips were dried by argon gas, and cleaned under O3 for 30 min to\u00a0 remove\u00a0 other\u00a0 impurities.\u00a0 After\u00a0 cleaning,\u00a0 the\u00a0 AFM\u00a0 tips\u00a0 were\u00a0 modified\u00a0 with\u00a0 3-aminopropyltriethoxysilane[17]\u00a0 to\u00a0 generate\u00a0 amino\u00a0 group,\u00a0 it\u00a0 was\u00a0 convenient\u00a0 for\u00a0 linking\u00a0 the\u00a0 heterobifunctional\u00a0 PEG\u00a0 (MAL-PEG2000-SCM,\u00a0 FW\u22482000, SensoPathechnologies, Bozeman, MT 1 mg mL\u22121). After drying with\u00a0 argon,\u00a0 the\u00a0 tips\u00a0 were\u00a0 immersed\u00a0 in\u00a0 a\u00a0 mixture\u00a0 of\u00a0 100\u00a0 \u00d7\u00a0 10\u22129m\u00a0 TDNs,\u00a0 50 \u03bcL\u00a0 NH2OH-reagent\u00a0 (500\u00a0 \u00d7\u00a0 10\u22123m\u00a0 NH2OH\u2022HCl,\u00a0 25\u00a0 \u00d7\u00a0 10\u22123m\u00a0 EDTA,\u00a0 pH\u00a0 7.5),\u00a0 and\u00a0 50\u00a0 \u03bcL\u00a0 buffer\u00a0 A\u00a0 (100\u00a0 \u00d7\u00a0 10\u22123m\u00a0 NaCl,\u00a0 50\u00a0 \u00d7\u00a0 10\u22123m\u00a0 NaH2PO4, 1 \u00d7 10\u22123m EDTA, pH 7.5). After functionalization for 1 h, the AFM tips were washed with PBS for three times and stored at 4 \u00b0C .*<\/p>\n\n\n\n *Xi Chen, Falin Tian, Min Li, Haijiao Xu, Mingjun Cai, Qian Li, Xiaolei Zuo, Hongda Wang, Xinghua Shi, Chunhai Fan, Huricha Baigude, Yuping Shan Please follow this external link to read the full article: https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/gch2.201900075 <\/a><\/p>\n\n\n\n Open Access: The article \u201cSize\u2010Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures<\/em>\u201d by Xi Chen, Falin Tian, Min Li, Haijiao Xu, Mingjun Cai, Qian Li, Xiaolei Zuo, Hongda Wang, Xinghua Shi, Chunhai Fan, Huricha Baigude and Yuping Shan is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/.<\/p>\n\n\n","protected":false},"excerpt":{"rendered":" Targeted drug delivery and precision medicine have now become a new paradigm in cancer therapy, with nanocarriers, pharmacologically active drugs could be directly delivered into target cancer cells to manage or reverse the course of disease. Nevertheless, the recent critical issue for drug delivery systems is lack of efficient drug delivery carriers.* DNA nanostructures have… Read More »Size\u2010Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":4380,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[16],"tags":[18,19,251,423,37,27,422,424,60,425,429,428,426],"class_list":["post-4377","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-afm-tips","tag-atomic-force-microscopy","tag-dna-nanostructures","tag-force-tracing","tag-pointprobe-plus","tag-pointprobe-plus-ppp","tag-ppp-bsi","tag-real-time-tracking","tag-scanning-probe-microscopy","tag-single-tetrahedral-dna-nanostructures","tag-tdn","tag-tdns","tag-transmembrane-kinetics"],"_links":{"self":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4377","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/comments?post=4377"}],"version-history":[{"count":0,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4377\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media\/4380"}],"wp:attachment":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media?parent=4377"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/categories?post=4377"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/tags?post=4377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}![\"\"](\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-1-from-Xi-Chen-et-al-2019-Size\u2010Independent-Transmembrane-Transporting-of-Single-Tetrahedral-DNA-Nanostructures-PPP-BSI-1024x405-2.jpg\"){kind=spacer}
Figure 1 from \u201cSize\u2010Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures<\/em>\u201d by Xi Chen et al:
Schematic diagram of the force tracing test. a) Scheme of assembling the TDNs, from left to right, ssDNA are strands A, B, C, and D (red, orange, green, and blue), respectively. The strand A stretch out a 5\u2010base ssDNA strand with sulfydryl group. The strand C is labeled with cyanine\u20103 (Cy3) fluorophores (Cy3\u2010TDNs). b) The diagram of connecting TDNs to the AFM tip, the TDNs is attached on the AFM tip with heterobifunctional PEG linker. c) The working principle of constant position mode force tracing technique. d) A typical force tracing curve with force signal (blue arrow). <\/figcaption><\/figure>\n\n\n\n
Size\u2010Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures<\/strong>
Global Challenges 2019, 1900075
DOI: https:\/\/doi.org\/10.1002\/gch2.201900075<\/p>\n\n\n\n