{"id":4319,"date":"2019-09-23T07:35:03","date_gmt":"2019-09-23T04:35:03","guid":{"rendered":"https:\/\/nanosensors.com\/blog\/nonlinear-biomechanical-characteristics-of-deep-deformation-of-native-rbc-membranes-in-normal-state-and-under-modifier-action\/"},"modified":"2023-03-15T14:51:40","modified_gmt":"2023-03-15T12:51:40","slug":"nonlinear-biomechanical-characteristics-of-deep-deformation-of-native-rbc-membranes-in-normal-state-and-under-modifier-action","status":"publish","type":"post","link":"https:\/\/www.nanosensors.com\/blog\/nonlinear-biomechanical-characteristics-of-deep-deformation-of-native-rbc-membranes-in-normal-state-and-under-modifier-action\/","title":{"rendered":"Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action"},"content":{"rendered":"\n<p>The mechanical properties and structural\norganization of membranes determine the functional state of red blood cells\n(RBCs). Deformability is one of the key physiological and biophysical\nindicators of RBC. Changes of the mechanical characteristics of cell membranes\ncan lead to a decrease in the rate of capillary blood flow and to development\nof stagnant phenomena in the microcirculation, and it can also reduce the\namount of oxygen delivered to the tissues.*<\/p>\n\n\n\n<p>In the article \u201cNonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action\u201d Elena Kozlova, Aleksandr Chernysh, Ekaterina Manchenko, Viktoria Sergunova and Viktor Moroz describe how they evaluated the ability of membranes of native human red blood cells (RBCs) to bend into the cell to a depth comparable in size with physiological deformations using the methods of atomic force microscopy ( AFM ) and atomic force spectroscopy ( AFS ).*<\/p>\n\n\n\n<p>As a true estimation of the elastic properties of RBC membranes can be obtained only by measurement of native cell properties the aim of the experiments was to study nonlinear mechanical characteristics of deep deformation of native RBC membranes in normal state and under the action of modifiers, in vitro to make sure that the result would be the closest to the characteristics of a living biological object.*<\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" aria-label=\"NANOSENSORS\u2122 rounded AFM tips of the type SD-R150-T3L450B (opens in a new tab)\" href=\"https:\/\/www.nanosensors.com\/pdf\/SpecialDevelopmentsList.pdf\" target=\"_blank\">NANOSENSORS\u2122 rounded AFM tips of the type SD-R150-T3L450B<\/a> with a typical tip radius of 150\u2009nm from the <a rel=\"noreferrer noopener\" aria-label=\"NANOSENSORS Special Developments List (opens in a new tab)\" href=\"https:\/\/www.nanosensors.com\/pdf\/SpecialDevelopmentsList.pdf\" target=\"_blank\">NANOSENSORS Special Developments List<\/a> were used to measure the deformation of the RBC membrane by atomic force spectroscopy ( AFS ).* <\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-5-2-c-from-Nonlinear-Biomechanical-Characteristics-of-Deep-Deformation-of-Native-RBC-Membranes-in-Normal-State-and-under-Modifier-Action-Elena-Kozlova-et-al_2-943x1024-2.jpg\" alt=\"\" class=\"wp-image-2044\"\/><figcaption><br> Figure 5.2. (c) from<em> \u201cNonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action \u201c <\/em>by Elena Kozlova et al.:<br> <em>\u00a0Bending of membranes under the action of force F <\/em>for stiff (1) and soft (2) membranes;<em> F <\/em>is the force acting on the membrane from the probe,<em> Z <\/em>is the vertical displacement of the piezoscanner<em>, h <\/em>is the depth of the membrane bending into RBC<em>, PBS <\/em>is the phosphate buffer solution, and<em> r<\/em>d<em> <\/em>is the bending radius of the membrane. <\/figcaption><\/figure>\n\n\n\n<p> *Elena Kozlova, Aleksandr Chernysh, Ekaterina Manchenko, Viktoria Sergunova, and Viktor Moroz<br><strong>Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action<\/strong><br> Scanning, Volume 2018, Article ID 1810585, 13 pages<br>Doi: https:\/\/doi.org\/10.1155\/2018\/1810585 <\/p>\n\n\n\n<p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\nPlease\nfollow this external link to read the full article: <a href=\"https:\/\/www.hindawi.com\/journals\/scanning\/2018\/1810585\/\">https:\/\/www.hindawi.com\/journals\/scanning\/2018\/1810585\/<\/a><\/p>\n\n\n\n<p>Open Access\nThe article \u00ab <em>Nonlinear Biomechanical Characteristics of Deep Deformation of\nNative RBC Membranes in Normal State and under Modifier Action<\/em> \u201d by Elena\nKozlova, Aleksandr Chernysh, Ekaterina Manchenko, Viktoria Sergunova, and\nViktor Moroz is licensed under a Creative Commons Attribution 4.0 International\nLicense, which permits use, sharing, adaptation, distribution and reproduction\nin any medium or format, as long as you give appropriate credit to the original\nauthor(s) and the source, provide a link to the Creative Commons license, and\nindicate if changes were made. The images or other third party material in this\narticle are included in the article\u2019s Creative Commons license, unless\nindicated otherwise in a credit line to the material. If material is not\nincluded in the article\u2019s Creative Commons license and your intended use is not\npermitted by statutory regulation or exceeds the permitted use, you will need\nto obtain permission directly from the copyright holder. To view a copy of this\nlicense, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The mechanical properties and structural organization of membranes determine the functional state of red blood cells (RBCs). Deformability is one of the key physiological and biophysical indicators of RBC. Changes of the mechanical characteristics of cell membranes can lead to a decrease in the rate of capillary blood flow and to development of stagnant phenomena&hellip;&nbsp;<a href=\"https:\/\/www.nanosensors.com\/blog\/nonlinear-biomechanical-characteristics-of-deep-deformation-of-native-rbc-membranes-in-normal-state-and-under-modifier-action\/\" class=\"\" rel=\"bookmark\">Read More &raquo;<span class=\"screen-reader-text\">Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":4322,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[16],"tags":[333,17,348,19,345,346,242,278,350,347,351,334,203,90,204,85,244,327,91],"class_list":["post-4319","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-afm-force-curves","tag-afm-probes","tag-afs","tag-atomic-force-microscopy","tag-atomic-force-spectroscopy","tag-biomechanics","tag-biophysics","tag-cell-biology","tag-cell-mechanics","tag-cell-membrane","tag-erythrocytes","tag-force-curves","tag-force-measurements","tag-force-spectroscopy","tag-hematology","tag-life-sciences","tag-membrane-biophysics","tag-nanoindentation","tag-rounded-afm-tips"],"_links":{"self":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4319","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/comments?post=4319"}],"version-history":[{"count":0,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4319\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media\/4322"}],"wp:attachment":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media?parent=4319"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/categories?post=4319"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/tags?post=4319"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}