{"id":4244,"date":"2019-03-24T22:07:54","date_gmt":"2019-03-24T20:07:54","guid":{"rendered":"https:\/\/nanosensors.com\/blog\/selective-oxidation-of-b800-bacteriochlorophyll-a-in-photosynthetic-light-harvesting-protein-lh2\/"},"modified":"2023-03-15T14:51:45","modified_gmt":"2023-03-15T12:51:45","slug":"selective-oxidation-of-b800-bacteriochlorophyll-a-in-photosynthetic-light-harvesting-protein-lh2","status":"publish","type":"post","link":"https:\/\/www.nanosensors.com\/blog\/selective-oxidation-of-b800-bacteriochlorophyll-a-in-photosynthetic-light-harvesting-protein-lh2\/","title":{"rendered":"Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2"},"content":{"rendered":"<p>Engineering chlorophyll (Chl) pigments that are bound to photosynthetic light-harvesting proteins is one promising strategy to regulate spectral coverage for photon capture and to improve the photosynthetic efficiency of these proteins.*<\/p>\n<p>The <i>in situ<\/i> oxidation of BChl <i>a<\/i> in light-harvesting protein LH2 from a purple bacterium <i>Rhodoblastus acidophilus<\/i> by 2,3-dichloro-5,6-dicyano-1,4-benzoquinone demonstrated in the article &#8220;<strong>Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2<\/strong>\u201d by Yoshitaka Saga et al. will be useful for engineering photofunctions in natural light-harvesting proteins and for understanding the alteration from BChl pigments in anoxygenic photosynthetic bacteria to Chl pigments in oxygenic organisms in the evolution of photosynthesis.*<\/p>\n<p>The authors observed their sample in 20\u2009mM Tris buffer containing 150\u2009mM NaCl (pH 8.0) using a\u00a0 home-built frequency modulation AFM ( FM-AFM ) with NANOSENSORS\u2122 <a href=\"https:\/\/www.nanosensors.com\/PointProbe-Plus-Non-Contact-Tapping-Mode-High-Resonance-Frequency-Au-coating-Detector-Side-afm-tip-PPP-NCHAuD\" target=\"_blank\" rel=\"noopener\">PPP-NCHAuD<\/a> AFM probes.*<\/p>\n<div id=\"attachment_1792\" style=\"width: 1210px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-6-from-Selective-oxidation-of-B800-bacteriochlorophyll-a-in-photosynthetic-light-harvesting-protein-LH2-2.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1792\" class=\"size-full wp-image-1792\" src=\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/figure-6-from-Selective-oxidation-of-B800-bacteriochlorophyll-a-in-photosynthetic-light-harvesting-protein-LH2-2.jpg\" alt=\"Figure 6 from \u00abSelective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2\u201d by Y. Saga et al.: FM-AFM images of native LH2 (A) and oxidized LH2 (B) adsorbed on mica taken in 20\u2009mM Tris buffer containing 150\u2009mM NaCl (pH 8.0). Left: wide images. Middle: locally enlarged images of single LH2 proteins. Right: overlapped height-profiles of ten proteins. NANOSENSORS PPP-NCHAuD AFM probes were used.\" width=\"1200\" height=\"832\" data-wp-pid=\"1792\" \/><\/a><p id=\"caption-attachment-1792\" class=\"wp-caption-text\">Figure 6 from \u00abSelective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2\u201d by Y. Saga et al.: FM-AFM images of native LH2 (A) and oxidized LH2 (B) adsorbed on mica taken in 20\u2009mM Tris buffer containing 150\u2009mM NaCl (pH 8.0). Left: wide images. Middle: locally enlarged images of single LH2 proteins. Right: overlapped height-profiles of ten proteins.<\/p><\/div>\n<p>*Yoshitaka Saga, Kiyoshiro Kawano, Yuji Otsuka, Michie Imanishi, Yukihiro Kimura, Sayaka Matsui &amp; Hitoshi Asakawa<br \/>\n<strong>Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2<\/strong><br \/>\nNature, Scientific Reports, volume 9, Article number: 3636 (2019)<br \/>\nDOI: https:\/\/doi.org\/10.1038\/s41598-019-40082-y<\/p>\n<p>Please follow this external link to read the full article: <a href=\"https:\/\/www.nature.com\/articles\/s41598-019-40082-y\" target=\"_blank\" rel=\"noopener\">https:\/\/www.nature.com\/articles\/s41598-019-40082-y<\/a><\/p>\n<p><b>Open Access<\/b> The article \u201cSelective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2\u201d by Yoshitaka Saga, Kiyoshiro Kawano, Yuji Otsuka, Michie Imanishi, Yukihiro Kimura, Sayaka Matsui &amp; Hitoshi Asakawa is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit <a href=\"http:\/\/creativecommons.org\/licenses\/by\/4.0\/\" target=\"_blank\" rel=\"noopener\">http:\/\/creativecommons.org\/licenses\/by\/4.0\/<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Engineering chlorophyll (Chl) pigments that are bound to photosynthetic light-harvesting proteins is one promising strategy to regulate spectral coverage for photon capture and to improve the photosynthetic efficiency of these proteins.* The in situ oxidation of BChl a in light-harvesting protein LH2 from a purple bacterium Rhodoblastus acidophilus by 2,3-dichloro-5,6-dicyano-1,4-benzoquinone demonstrated in the article &#8220;Selective&hellip;&nbsp;<a href=\"https:\/\/www.nanosensors.com\/blog\/selective-oxidation-of-b800-bacteriochlorophyll-a-in-photosynthetic-light-harvesting-protein-lh2\/\" class=\"\" rel=\"bookmark\">Read More &raquo;<span class=\"screen-reader-text\">Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":4245,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[16],"tags":[82,17,19,265,266,268,267,130,129,270,264,269,37,27,263,60],"class_list":["post-4244","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-afm-cantilever","tag-afm-probes","tag-atomic-force-microscopy","tag-chemical-modification","tag-fm-afm","tag-frequency-modulation-atomic-force-microscopy","tag-frrequency-modulation-afm","tag-gold-coated-afm-tips","tag-gold-coated-pointprobe-plus-afm-tips","tag-light-harvesting","tag-membrane-proteins","tag-photosynthesis","tag-pointprobe-plus","tag-pointprobe-plus-ppp","tag-ppp-nchaud","tag-scanning-probe-microscopy"],"_links":{"self":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4244","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/comments?post=4244"}],"version-history":[{"count":0,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4244\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media\/4245"}],"wp:attachment":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media?parent=4244"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/categories?post=4244"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/tags?post=4244"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}