![\"Figure](\"https:\/\/nanosensors.com\/blog\/wp-content\/uploads\/2022\/11\/Figure-5-from-5\u2032-CGAn-sequence-assisted-pH-controlled-assembly-of-supramolecular-DNA-nanostructure-2.jpg\")
<\/a>Figure 5. from \u201c5\u2032-(CGA)n sequence-assisted pH-controlled assembly of supramolecular DNA nanostructure\u201d by Yuting Yan et al.: AFM images of the nanostructures formed by DNA G-quadruplexes self-assembly in KOAc buffer solution; (a,b) SG2 at pH 9.0, (c,d) SG2 at pH 4.5, (e,f) a mixture of SG2 and CSG2 at pH 4.5, (g,h) a mixture of SG2 and CSG2 at pH 9.0. The length of side is 2\u2009\u00b5m and the scale bar is 500\u2009nm.<\/p><\/div>\n
AFM microscopy was performed on the fresh mica surfaces with the help of magnesium ions which can bind negatively charged DNA strands. The DNA samples were annealed at 100\u2009\u00b5M in 100\u2009mM K+ solution at 4\u00b0C for one week. Then aliquots were diluted with 2\u2009mM MgCl2 aqueous solution to give a 20\u2009\u00b5l analyte containing 1.5\u2009\u00b5M DNA. The analytes were spread evenly on the mica surface for 5\u20138\u2009min. Subsequently, the mica surface was washed with Milli-Q water to wipe off the excess salt, and finally dried in the air.*<\/p>\n
*Yuting Yan, Yanwei Cao, Chunsheng Xiao, Yang Li, Xiaoxuan Xiang, Xinhua Guo Open Access: The article “5\u2032-(CGA)n sequence-assisted pH-controlled assembly of supramolecular DNA nanostructure” by\u00a0 Yuting Yan et al. is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article\u2019s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http:\/\/creativecommons.org\/licenses\/by\/4.0\/.<\/p>\n","protected":false},"excerpt":{"rendered":" In the research article “5\u2032-(CGA)n sequence-assisted pH-controlled assembly of supramolecular DNA nanostructure” Yuting Yan, Yanwei Cao, Chunsheng Xiao, Yang Li, Xiaoxuan Xiang and Xinhua Guo demonstratethat the connection of duplex-forming sequences with a G-quadruplex-forming sequence (G6) could be used to construct DNA supramolecular nanostructures with alternating B-duplex and G-quadruplex structures. Their results demonstrate that the… Read More »5\u2032-(CGA)n sequence-assisted pH-controlled assembly of supramolecular DNA nanostructure<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":4226,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[16],"tags":[17,18,19,248,252,247,251,253,115,164,249,37,27,246,60,109,250,114],"class_list":["post-4225","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-afm-probes","tag-afm-tips","tag-atomic-force-microscopy","tag-cga-dna-sequence","tag-dna","tag-dna-assembly","tag-dna-nanostructures","tag-nanostructures","tag-non-contact","tag-non-contact-afm-probes","tag-ph-controlled-dna-nanostructure","tag-pointprobe-plus","tag-pointprobe-plus-ppp","tag-ppp-nchr","tag-scanning-probe-microscopy","tag-spm-probes","tag-supramolecular-dna-nanostructures","tag-tapping-mode"],"_links":{"self":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4225","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/comments?post=4225"}],"version-history":[{"count":0,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4225\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media\/4226"}],"wp:attachment":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media?parent=4225"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/categories?post=4225"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/tags?post=4225"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n5\u2032-(CGA)n sequence-assisted pH-controlled assembly of supramolecular DNA nanostructure
\n<\/strong>Royal Society Open Science, 1 August 2018, Volume 5, Issue 8
\nDOI: https:\/\/doi.org\/10.1098\/rsos.180123<\/a><\/p>\n