{"id":4179,"date":"2018-08-15T11:39:20","date_gmt":"2018-08-15T08:39:20","guid":{"rendered":"https:\/\/nanosensors.com\/blog\/zn2-triggered-self-assembly-of-gonadorelin-6-d-phe-to-produce-nanostructures-and-fibrils\/"},"modified":"2023-03-15T14:53:14","modified_gmt":"2023-03-15T12:53:14","slug":"zn2-triggered-self-assembly-of-gonadorelin-6-d-phe-to-produce-nanostructures-and-fibrils","status":"publish","type":"post","link":"https:\/\/www.nanosensors.com\/blog\/zn2-triggered-self-assembly-of-gonadorelin-6-d-phe-to-produce-nanostructures-and-fibrils\/","title":{"rendered":"Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils"},"content":{"rendered":"

For this article the AFM images taken with tapping mode in Tris buffer solution were performed with the NANOSENSORS qp-BioAC<\/a> (cantilever 3, resonance frequency 30kHz).<\/p>\n

\"Figure<\/a>

Figure 5 from Yordanova et. al. “Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils<\/a>” – AFM image of the Zn2+: GnRH [6-D-Phe] 10:1 complex. (a,b) Oligomers after preparation with tapping mode in Tris buffer solution (c,d) fibrils with tapping mode in air (z-scale indicates the average size of the formed oligomers and fibrils).<\/p><\/div>\n

Yordanka Yordanova, Willem Vanderlinden, Raphael Stoll, Daniel R\u00fcdiger, Andreas Tosstorff, Wolfgang Zaremba, Gerhard Winter, Stefan Zahler & Wolfgang Friess<\/strong>
\nZn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils<\/em>
\nNature Scientific Reports volume 8, Article number: 11280 (2018)
\ndoi: https:\/\/doi.org\/10.1038\/s41598-018-29529-w<\/a>
\nPlease follow this external link to read the full article on the\u00a0 Nature website: https:\/\/rdcu.be\/4y9J<\/a><\/p>\n

Abstract: A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+<\/sup> in vitro<\/i>. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR\u2013FTIR) revealed the existence of higher order assembly of Zn2+<\/sup>: GnRH [6-D-Phe]. Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+<\/sup> and GnRH [6-D-Phe]. Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils. Molecular Dynamic (MD) simulation of the 10:1 Zn2+<\/sup>: GnRH [6-D-Phe] explored the interaction and dimerization processes. In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift. The lyophilized Zn2+<\/sup>: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices. The in vitro<\/i> release was slow and continuous over 14 days and not influenced by the oil matrix.<\/p>\n

The article “Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils<\/a>” by Yordanova Y. et al<\/i>. is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit https:\/\/creativecommons.org\/licenses\/by\/4.0\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

For this article the AFM images taken with tapping mode in Tris buffer solution were performed with the NANOSENSORS qp-BioAC (cantilever 3, resonance frequency 30kHz).   Yordanka Yordanova, Willem Vanderlinden, Raphael Stoll, Daniel R\u00fcdiger, Andreas Tosstorff, Wolfgang Zaremba, Gerhard Winter, Stefan Zahler & Wolfgang Friess Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils… Read More »Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":4180,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[16],"tags":[17,18,19,84,85,110,182,253,181,60,183,50],"class_list":["post-4179","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-science-technology","tag-afm-probes","tag-afm-tips","tag-atomic-force-microscopy","tag-biology","tag-life-sciences","tag-microcantilevers","tag-nanoparticles","tag-nanostructures","tag-qp-bioac","tag-scanning-probe-microscopy","tag-self-assembly","tag-uniqprobe"],"_links":{"self":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4179","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/comments?post=4179"}],"version-history":[{"count":0,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/posts\/4179\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media\/4180"}],"wp:attachment":[{"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/media?parent=4179"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/categories?post=4179"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nanosensors.com\/blog\/wp-json\/wp\/v2\/tags?post=4179"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}