Tag Archives: microcantilevers

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils

For this article the AFM images taken with tapping mode in Tris buffer solution were performed with the NANOSENSORS qp-BioAC (cantilever 3, resonance frequency 30kHz).

Figure 5 from Yordanova et. al. "Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils" - AFM image of the Zn2+: GnRH [6-D-Phe] 10:1 complex. (a,b) Oligomers after preparation with tapping mode in Tris buffer solution (c,d) fibrils with tapping mode in air (z-scale indicates the average size of the formed oligomers and fibrils). NANOSENSORS qp-BioAC AFM probe was used to perform images in buffer solution
Figure 5 from Yordanova et. al. “Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils” – AFM image of the Zn2+: GnRH [6-D-Phe] 10:1 complex. (a,b) Oligomers after preparation with tapping mode in Tris buffer solution (c,d) fibrils with tapping mode in air (z-scale indicates the average size of the formed oligomers and fibrils).

Yordanka Yordanova, Willem Vanderlinden, Raphael Stoll, Daniel Rüdiger, Andreas Tosstorff, Wolfgang Zaremba, Gerhard Winter, Stefan Zahler & Wolfgang Friess
Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils
Nature Scientific Reports volume 8, Article number: 11280 (2018)
doi: https://doi.org/10.1038/s41598-018-29529-w
Please follow this external link to read the full article on the  Nature website: https://rdcu.be/4y9J

Abstract: A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+ in vitro. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR–FTIR) revealed the existence of higher order assembly of Zn2+: GnRH [6-D-Phe]. Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+ and GnRH [6-D-Phe]. Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils. Molecular Dynamic (MD) simulation of the 10:1 Zn2+: GnRH [6-D-Phe] explored the interaction and dimerization processes. In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift. The lyophilized Zn2+: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices. The in vitro release was slow and continuous over 14 days and not influenced by the oil matrix.

The article “Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils” by Yordanova Y. et al. is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/

New Self-sensing Self-actuating Akiyama-probe Website Uploaded

NANOSENSORS self-sensing and self-actuating Akiyama-probe AFM probe
NANOSENSORS self-sensing and self-actuating Akiyama-probe

We have uploaded an updated version of the NANOSENSORS Akiyama-probe webpage. On the new website you will find information not only about how this self-sensing and self-actuating probe for Atomic Force Microscopy works but also about the required set-up and electrical configuration and a lot more.

Please have a look at: www.akiyamaprobe.com

探針がカンチレバー先端より突出し、直上から探針先端を観察可能。NANOSENSORS アドバンスドテック AdvancedTEC™ (ATEC) プローブ

アドバンスドテックシリーズはシャープな三角錐の探針がカンチレバー先端より更に前方へと突き出ているのが特長。

探針を確実に測定領域にポジショニングしたい場合や、探針先端を直接見ながら作業するナノマニピュレーションなどのアプリケーションに最適です。

Screencasts on the NANOSENSORS AdvancedTEC  tip view AFM probes are also available in English: