Tag Archives: AFM cantilever

Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2

Engineering chlorophyll (Chl) pigments that are bound to photosynthetic light-harvesting proteins is one promising strategy to regulate spectral coverage for photon capture and to improve the photosynthetic efficiency of these proteins.*

The in situ oxidation of BChl a in light-harvesting protein LH2 from a purple bacterium Rhodoblastus acidophilus by 2,3-dichloro-5,6-dicyano-1,4-benzoquinone demonstrated in the article “Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2” by Yoshitaka Saga et al. will be useful for engineering photofunctions in natural light-harvesting proteins and for understanding the alteration from BChl pigments in anoxygenic photosynthetic bacteria to Chl pigments in oxygenic organisms in the evolution of photosynthesis.*

The authors observed their sample in 20 mM Tris buffer containing 150 mM NaCl (pH 8.0) using a  home-built frequency modulation AFM ( FM-AFM ) with NANOSENSORS™ PPP-NCHAuD AFM probes.*

Figure 6 from «Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2” by Y. Saga et al.: FM-AFM images of native LH2 (A) and oxidized LH2 (B) adsorbed on mica taken in 20 mM Tris buffer containing 150 mM NaCl (pH 8.0). Left: wide images. Middle: locally enlarged images of single LH2 proteins. Right: overlapped height-profiles of ten proteins. NANOSENSORS PPP-NCHAuD AFM probes were used.
Figure 6 from «Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2” by Y. Saga et al.: FM-AFM images of native LH2 (A) and oxidized LH2 (B) adsorbed on mica taken in 20 mM Tris buffer containing 150 mM NaCl (pH 8.0). Left: wide images. Middle: locally enlarged images of single LH2 proteins. Right: overlapped height-profiles of ten proteins.

*Yoshitaka Saga, Kiyoshiro Kawano, Yuji Otsuka, Michie Imanishi, Yukihiro Kimura, Sayaka Matsui & Hitoshi Asakawa
Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2
Nature, Scientific Reports, volume 9, Article number: 3636 (2019)
DOI: https://doi.org/10.1038/s41598-019-40082-y

Please follow this external link to read the full article: https://www.nature.com/articles/s41598-019-40082-y

Open Access The article “Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2” by Yoshitaka Saga, Kiyoshiro Kawano, Yuji Otsuka, Michie Imanishi, Yukihiro Kimura, Sayaka Matsui & Hitoshi Asakawa is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Get free NANOSENSORS qp-fast AFM probes at booth 613 at APS March 2019

Are you looking for AFM probes with unsurpassed small variation in spring constant and resonance frequency? Then visit the NanoAndMore USA booth no. 613 at the 2019 APS March Exhibit and get your free sample of NANOSENSORS™ uniqprobe qp-fast AFM probes.

Please have a look at the uniqprobe brochure or the uniqprobe video on Youtube if you would like to learn more about this AFM probes series.

NANOSENSORS uniqprobe qp-fast AFM probe
NANOSENSORS uniqprobe qp-fast AFM probe

Get free NANOSENSORS qp-fast AFM probes at booth 610 at MRS Fall 2018

Are you looking for AFM probes with unsurpassed small variation in spring constant and resonance frequency? Then visit the NanoAndMore USA booth no. 610 at the 2018 MRS Fall Exhibit and get your free sample of NANOSENSORS™ uniqprobe qp-fast AFM probes.

NANOSENSORS uniqprobe qp-fast AFM probe
NANOSENSORS uniqprobe qp-fast AFM probe